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TEP1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Telomerase associated protein 1

Gene Aliases

P240; p80 telomerase homolog; telomerase protein 1; telomerase protein component 1; Telomerase-associated protein 1; TLP1; TP1; TROVE domain family, member 1; TROVE1; VAULT2.

Gene ID

7011

Accession Number

NP_009041.2

Reactivity

Homo sapiens, Human

Target

Telomerase protein component 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology TEP1 ELISA Kit (Human) (OKEH00524) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-dog TEP1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TEP1 and incubated. The TEP1 found in the sample or standards competes with the biotinylated TEP1 for limited binding sites on the immobilized anti-dog TEP1 antibody. Excess unbound biotinylated TEP1 and sample or standard TEP1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TEP1 captured in the well and inversely proportional to the amount of TEP1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.067 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

290 kDa

Specificity

Natural and recombinant Human Telomerase protein component 1

Protein Length

2627

NCBI Gene Symbol

TEP1

Protein Name

Q99973

Gene Name URL

TEP1

Nucleotide Accession Number

NM_007110.4

Frequently Asked Questions

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