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MMP9 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Matrix metallopeptidase 9

Gene Aliases

92 kDa gelatinase;92 kDa type IV collagenase;92-kDa type IV collagenase; gelatinase B; GELB; matrix metalloproteinase 9 (gelatinase B, 92-kDa type IV collagenase) ; matrix metalloproteinase-9; MMP-9.

Gene ID

81687

Accession Number

NP_112317.1

Reactivity

Rat, Rattus norvegicus

Target

Matrix metalloproteinase-9

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MMP9 ELISA Kit (Rat) (OKEH00522) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Rat MMP9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Rat MMP9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Rat MMP9 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.433 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

79 kDa

Specificity

Natural and recombinant Rat Matrix metalloproteinase-9

Protein Length

708

NCBI Gene Symbol

Mmp9

Protein Name

P50282

Gene Name URL

Mmp9

Nucleotide Accession Number

NM_031055.1

Frequently Asked Questions

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