What is the catalog number for NPPB ELISA Kit (Human)?

The catalog number for NPPB ELISA Kit (Human) is OKEH00508-96W.

What sizes are available for NPPB ELISA Kit (Human)?

NPPB ELISA Kit (Human) is available in the following size(s): 96 Well.

Is NPPB ELISA Kit (Human) in stock?

NPPB ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

NPPB ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Natriuretic peptide B

Gene Aliases

BNP; brain natriuretic factor prohormone; brain type natriuretic peptide; gamma-brain natriuretic peptide; Iso-ANP; natriuretic peptide precursor B; natriuretic peptides B; natriuretic protein; preproBNP; proBNP.

Gene ID

4879

Accession Number

NP_002512.1

Reactivity

Homo sapiens, Human

Target

Natriuretic peptides B

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NPPB ELISA Kit (Human) (OKEH00508) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-dog NPPB antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated NPPB and incubated. The NPPB found in the sample or standards competes with the biotinylated NPPB for limited binding sites on the immobilized anti-dog NPPB antibody. Excess unbound biotinylated NPPB and sample or standard NPPB is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated NPPB captured in the well and inversely proportional to the amount of NPPB which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol