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LGALS3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Galectin 3

Gene Aliases

35 kDa lectin; advanced glycation end-product receptor 3; carbohydrate-binding protein 35; CBP35; epididymis secretory sperm binding protein; GAL3; galactose-specific lectin 3; Galactoside-binding protein; GALBP; galectin-3; GALIG; IgE-binding protein; L31; L-31; laminin-binding protein; lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; MAC2; MAC-2 antigen.

Gene ID

3958

Accession Number

NP_002297.2

Reactivity

Homo sapiens, Human

Target

Galectin-3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LGALS3 ELISA Kit (Human) (OKEH00477) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human LGALS3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Human LGALS3 and incubated. The Human LGALS3 found in the sample or standards competes with the biotinylated Human LGALS3 for limited binding sites on the immobilized anti-Human LGALS3 antibody. Excess unbound biotinylated Human LGALS3 and sample or standard Human LGALS3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Human LGALS3 captured in the well and inversely proportional to the amount of Human LGALS3 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

62.5-4000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

30 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

26 kDa

Specificity

Natural and recombinant Human Galectin-3

Protein Length

250

NCBI Gene Symbol

LGALS3

Protein Name

P17931

Gene Name URL

LGALS3

Nucleotide Accession Number

NM_002306.3

Frequently Asked Questions

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