LGALS3 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Galectin 3
Gene Aliases
35 kDa lectin; advanced glycation end-product receptor 3; carbohydrate-binding protein 35; CBP35; epididymis secretory sperm binding protein; GAL3; galactose-specific lectin 3; Galactoside-binding protein; GALBP; galectin-3; GALIG; IgE-binding protein; L31; L-31; laminin-binding protein; lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; MAC2; MAC-2 antigen.
Gene ID
3958
Accession Number
NP_002297.2
Reactivity
Homo sapiens, Human
Target
Galectin-3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LGALS3 ELISA Kit (Human) (OKEH00477) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human LGALS3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Human LGALS3 and incubated. The Human LGALS3 found in the sample or standards competes with the biotinylated Human LGALS3 for limited binding sites on the immobilized anti-Human LGALS3 antibody. Excess unbound biotinylated Human LGALS3 and sample or standard Human LGALS3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Human LGALS3 captured in the well and inversely proportional to the amount of Human LGALS3 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
62.5-4000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
30 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
26 kDa
Specificity
Natural and recombinant Human Galectin-3
Protein Length
250
NCBI Gene Symbol
LGALS3
Protein Name
P17931
Gene Name URL
LGALS3
Nucleotide Accession Number
NM_002306.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog