What is the catalog number for TSHB ELISA Kit (Human)?

The catalog number for TSHB ELISA Kit (Human) is OKEH00458-96W.

What sizes are available for TSHB ELISA Kit (Human)?

TSHB ELISA Kit (Human) is available in the following size(s): 96 Well.

Is TSHB ELISA Kit (Human) in stock?

TSHB ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Does this product need any special customs documentation to be imported to my country?

If you’re outside the EU, UK, or USA, you’re responsible for customs clearance and any related taxes or fees. We provide the standard import documents, such as the Commercial Invoice, Packing List, and Airway Bill, in advance, so let us know early if your customs office needs anything additional for this specific product.

What buffer is this product supplied in?

The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

TSHB ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Thyroid stimulating hormone subunit beta

Gene Aliases

Thyroid stimulating hormone beta; Thyroid-stimulating hormone subunit beta; thyrotropin beta chain; thyrotropin subunit beta; TSH-B; TSH-BETA.

Gene ID

7252

Accession Number

NP_000540.2

Reactivity

Homo sapiens, Human

Target

Thyrotropin subunit beta

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TSHB ELISA Kit (Human) (OKEH00458) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human TSHB antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Human TSHB and incubated. The Human TSHB found in the sample or standards competes with the biotinylated Human TSHB for limited binding sites on the immobilized anti-Human TSHB antibody. Excess unbound biotinylated Human TSHB and sample or standard Human TSHB is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Human TSHB captured in the well and inversely proportional to the amount of Human TSHB which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol