INS ELISA Kit (Dog)
Product Specifications
CAS Number
7732-18-5
Gene Name
Insulin
Gene Aliases
Insulin.
Gene ID
483665
Accession Number
NP_001123565.1
Reactivity
Canis lupus, Dog
Target
Insulin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology INS ELISA Kit (Dog) (OKEH00457) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dog INS antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dog INS and incubated. The Dog INS found in the sample or standards competes with the biotinylated Dog INS for limited binding sites on the immobilized anti-Dog INS antibody. Excess unbound biotinylated Dog INS and sample or standard Dog INS is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dog INS captured in the well and inversely proportional to the amount of Dog INS which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50mIU/L
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
Sensitivity
0.19mIU/L
Reconstitution
Store as indicated in product manual.
Molecular Weight
12 kDa
Specificity
Natural and recombinant Dog Insulin
Protein Length
110
NCBI Gene Symbol
INS
Protein Name
P01321
Gene Name URL
INS
Nucleotide Accession Number
NM_001130093.1
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