What is the catalog number for INS ELISA Kit (Dog)?

The catalog number for INS ELISA Kit (Dog) is OKEH00457-96W.

What sizes are available for INS ELISA Kit (Dog)?

INS ELISA Kit (Dog) is available in the following size(s): 96 Well.

Is INS ELISA Kit (Dog) in stock?

INS ELISA Kit (Dog) is currently made to order / not held in immediate stock. Contact us for lead time.

INS ELISA Kit (Dog)

Product Specifications

CAS Number

7732-18-5

Gene Name

Insulin

Gene Aliases

Insulin.

Gene ID

483665

Accession Number

NP_001123565.1

Reactivity

Canis lupus, Dog

Target

Insulin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology INS ELISA Kit (Dog) (OKEH00457) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dog INS antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dog INS and incubated. The Dog INS found in the sample or standards competes with the biotinylated Dog INS for limited binding sites on the immobilized anti-Dog INS antibody. Excess unbound biotinylated Dog INS and sample or standard Dog INS is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dog INS captured in the well and inversely proportional to the amount of Dog INS which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol