What is the catalog number for INS ELISA Kit (Human)?

The catalog number for INS ELISA Kit (Human) is OKEH00456-96W.

What sizes are available for INS ELISA Kit (Human)?

INS ELISA Kit (Human) is available in the following size(s): 96 Well.

Is INS ELISA Kit (Human) in stock?

INS ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

What is the difference between this product and a similar catalogue number?

Differences typically involve clone, conjugate, host species, or validated applications. Compare the respective datasheets, or contact us with both catalogue numbers for a direct comparison.

INS ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Insulin

Gene Aliases

IDDM; IDDM1; IDDM2; ILPR; insulin; IRDN; MODY10; PNDM4; preproinsulin; proinsulin.

Gene ID

3630

Accession Number

NP_000198.1

Reactivity

Homo sapiens, Human

Target

Insulin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology INS ELISA Kit (Human) (OKEH00456) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human INS antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated INS and incubated. The INS found in the sample or standards competes with the biotinylated INS for limited binding sites on the immobilized anti-Human INS antibody. Excess unbound biotinylated INS and sample or standard INS is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated INS captured in the well and inversely proportional to the amount of INS which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol