What is the catalog number for C-Peptide ELISA Kit (Mouse)?

The catalog number for C-Peptide ELISA Kit (Mouse) is OKEH00454-96W.

What sizes are available for C-Peptide ELISA Kit (Mouse)?

C-Peptide ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is C-Peptide ELISA Kit (Mouse) in stock?

C-Peptide ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.

Do you ship this product with dry ice?

Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.

C-Peptide ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Aliases

C Peptide, C Peptide, Proinsulin, C-Peptide, C-Peptide, Proinsulin , Connecting Peptide, Proinsulin C Peptide, Proinsulin C-Peptide

Reactivity

Mouse

Target

C-peptide is a protein that is produced in the body along with insulin. First preproinsulin is secreted with an A-chain, C-peptide, a B-chain, and a signal sequence. The signal sequence is cut off, leaving proinsulin. Then the C-peptide is cut off, leaving the A-chain and B-chain to form insulin.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology C-Peptide ELISA Kit (Mouse) (OKEH00454) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse C-Peptide antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Mouse C-Peptide and incubated. The Mouse C-Peptide found in the sample or standards competes with the biotinylated Mouse C-Peptide for limited binding sites on the immobilized anti-Mouse C-Peptide antibody. Excess unbound biotinylated Mouse C-Peptide and sample or standard Mouse C-Peptide is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Mouse C-Peptide captured in the well and inversely proportional to the amount of Mouse C-Peptide which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol