What is the catalog number for C-Peptide ELISA Kit (Human)?

The catalog number for C-Peptide ELISA Kit (Human) is OKEH00453-96W.

What sizes are available for C-Peptide ELISA Kit (Human)?

C-Peptide ELISA Kit (Human) is available in the following size(s): 96 Well.

Is C-Peptide ELISA Kit (Human) in stock?

C-Peptide ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

C-Peptide ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Aliases

C Peptide, C Peptide, Proinsulin, C-Peptide, C-Peptide, Proinsulin , Connecting Peptide, Proinsulin C Peptide, Proinsulin C-Peptide

Reactivity

Human

Target

C-peptide is a protein that is produced in the body along with insulin. First preproinsulin is secreted with an A-chain, C-peptide, a B-chain, and a signal sequence. The signal sequence is cut off, leaving proinsulin. Then the C-peptide is cut off, leaving the A-chain and B-chain to form insulin.

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology C-Peptide ELISA Kit (Human) (OKEH00453) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human C-Peptide antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Human C-Peptide and incubated. The Human C-Peptide found in the sample or standards competes with the biotinylated Human C-Peptide for limited binding sites on the immobilized anti-Human C-Peptide antibody. Excess unbound biotinylated Human C-Peptide and sample or standard Human C-Peptide is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Human C-Peptide captured in the well and inversely proportional to the amount of Human C-Peptide which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol