CGB ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Chorionic gonadotropin subunit beta 3, chorionic gonadotropin subunit beta 5, chorionic gonadotropin subunit beta 8
Gene Aliases
CGB; CGB5; CGB7; CGB8; CG-beta; Choriogonadotropin subunit beta; choriogonadotropin subunit beta 3; chorionic gonadotrophin chain beta; chorionic gonadotropin beta 3 subunit; chorionic gonadotropin beta 5 subunit; chorionic gonadotropin beta 8 subunit; chorionic gonadotropin beta chain; chorionic gonadotropin beta subunit 3; chorionic gonadotropin beta subunit 5; chorionic gonadotropin beta subunit 8; chorionic gonadotropin chain beta; chorionic gonadotropin, beta polypeptide; chorionic gonadotropin, beta polypeptide 5; chorionic gonadotropin, beta polypeptide 8; HCG; hCGB; LHB; luteinizing hormone beta subunit.
Gene ID
1082, 93659, 94115
Accession Number
NP_000728
Reactivity
Homo sapiens, Human
Target
Choriogonadotropin subunit beta 3
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CGB ELISA Kit (Human) (OKEH00451) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CGB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human CGB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CGB captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
0.35 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
17 kDa
Specificity
Natural and recombinant Human Choriogonadotropin subunit beta
Protein Length
165
NCBI Gene Symbol
CGB3, CGB5, CGB8
Protein Name
P0DN86
Gene Name URL
CGB3, CGB5, CGB8
Nucleotide Accession Number
NM_000737
Frequently Asked Questions
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