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CGA ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Glycoprotein hormones, alpha polypeptide

Gene Aliases

Anterior pituitary glycoprotein hormones common subunit alpha; CG-ALPHA; choriogonadotropin alpha chain; chorionic gonadotrophin subunit alpha; chorionic gonadotropin, alpha polypeptide; follicle-stimulating hormone alpha chain; follicle-stimulating hormone alpha subunit; follitropin alpha chain; FSHA; FSH-alpha; glycoprotein hormones alpha chain; GPA1; GPHa; GPHA1; HCG; LHA; LSH-alpha; luteinizing hormone alpha chain; lutropin alpha chain; thyroid-stimulating hormone alpha chain; thyrotropin alpha chain; TSHA; TSH-alpha.

Gene ID

1081

Accession Number

NP_000726.1

Reactivity

Homo sapiens, Human

Target

Glycoprotein hormones alpha chain

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CGA ELISA Kit (Human) (OKEH00449) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CGA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human CGA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CGA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

3.75-240mIU/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%

Sensitivity

1.4mIU/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

13 kDa

Specificity

Natural and recombinant Human Glycoprotein hormones alpha chain

Protein Length

116

NCBI Gene Symbol

CGA

Protein Name

P01215

Gene Name URL

CGA

Nucleotide Accession Number

NM_000735.3

Frequently Asked Questions

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