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SOX18 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

SRY-box transcription factor 18

Gene Aliases

HLTRS; HLTS; SRY (sex determining region Y) -box 18; SRY-box 18; transcription factor SOX-18.

Gene ID

54345

Accession Number

NP_060889.1

Reactivity

Homo sapiens, Human

Target

Transcription factor SOX-18

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SOX18 ELISA Kit (Human) (OKEH00435) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human SOX18 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human SOX18 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human SOX18 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.056 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

40 kDa

Specificity

Natural and recombinant Human Transcription factor SOX-18

Protein Length

384

NCBI Gene Symbol

SOX18

Protein Name

P35713

Gene Name URL

SOX18

Nucleotide Accession Number

NM_018419.2

Frequently Asked Questions

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