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PLA2G6 ELISA Kit (Human)

Selected Variant

OKEH00433-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Phospholipase A2 group VI

Gene Aliases

2-lysophosphatidylcholine acylhydrolase;85 kDa calcium-independent phospholipase A2;85/88 kDa calcium-independent phospholipase A2; CaI-PLA2; Group VI phospholipase A2; GVI; GVI PLA2; INAD1; intracellular membrane-associated calcium-independent phospholipase A2 beta; iPLA2; iPLA2beta; iPLA2-beta; IPLA2-VIA; NBIA2; NBIA2A; NBIA2B; neurodegeneration with brain iron accumulation 2; palmitoyl-CoA hydrolase; PARK14; patatin-like phospholipase domain-containing protein 9; phospholipase A2, group VI (cytosolic, calcium-independent) ; PLA2; PNPLA9.

Gene ID

8398

Accession Number

NP_001004426.1

Reactivity

Homo sapiens, Human

Target

85/88 kDa calcium-independent phospholipase A2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PLA2G6 ELISA Kit (Human) (OKEH00433) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human PLA2G6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human PLA2G6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human PLA2G6 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.625-40ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.28 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

89 kDa

Specificity

Natural and recombinant Human 85/88 kDa calcium-independent phospholipase A2

Protein Length

806

NCBI Gene Symbol

PLA2G6

Protein Name

O60733

Gene Name URL

PLA2G6

Nucleotide Accession Number

NM_001004426.1

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