PLA2G6 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Phospholipase A2 group VI
Gene Aliases
2-lysophosphatidylcholine acylhydrolase;85 kDa calcium-independent phospholipase A2;85/88 kDa calcium-independent phospholipase A2; CaI-PLA2; Group VI phospholipase A2; GVI; GVI PLA2; INAD1; intracellular membrane-associated calcium-independent phospholipase A2 beta; iPLA2; iPLA2beta; iPLA2-beta; IPLA2-VIA; NBIA2; NBIA2A; NBIA2B; neurodegeneration with brain iron accumulation 2; palmitoyl-CoA hydrolase; PARK14; patatin-like phospholipase domain-containing protein 9; phospholipase A2, group VI (cytosolic, calcium-independent) ; PLA2; PNPLA9.
Gene ID
8398
Accession Number
NP_001004426.1
Reactivity
Homo sapiens, Human
Target
85/88 kDa calcium-independent phospholipase A2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PLA2G6 ELISA Kit (Human) (OKEH00433) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human PLA2G6 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human PLA2G6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human PLA2G6 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.28 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
89 kDa
Specificity
Natural and recombinant Human 85/88 kDa calcium-independent phospholipase A2
Protein Length
806
NCBI Gene Symbol
PLA2G6
Protein Name
O60733
Gene Name URL
PLA2G6
Nucleotide Accession Number
NM_001004426.1
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS6153958-01 0.1 mL
PAK1 (Serine/Threonine-protein Kinase PAK 1, Alpha-PAK, p21-activated Kinase 1, PAK-1, p65-PAK, MGC130000, MGC130001) (HRP)
Ask
View Details 
MBS6153958-02 5x 0.1 mL
PAK1 (Serine/Threonine-protein Kinase PAK 1, Alpha-PAK, p21-activated Kinase 1, PAK-1, p65-PAK, MGC130000, MGC130001) (HRP)
Ask
View Details 