What is the catalog number for APH1A ELISA Kit (Human)?

The catalog number for APH1A ELISA Kit (Human) is OKEH00431-96W.

What sizes are available for APH1A ELISA Kit (Human)?

APH1A ELISA Kit (Human) is available in the following size(s): 96 Well.

Is APH1A ELISA Kit (Human) in stock?

APH1A ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Can you help me confirm if this product is right for my specific experiment?

Yes, contact us with details of your sample type, species, and intended application, and our specialists will confirm suitability or suggest an alternative from our supplier network if this isn’t the best fit.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

APH1A ELISA Kit (Human)

Selected Variant

OKEH00431-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Aph-1 homolog A, gamma-secretase subunit

Gene Aliases

6530402N02Rik; anterior pharynx defective 1 homolog A; APH-1; APH-1A; APH1A gamma secretase subunit; aph-1alpha; CGI-78; gamma-secretase subunit APH-1A; presenilin-stabilization factor.

Gene ID

51107

Accession Number

NP_001071096.1

Reactivity

Homo sapiens, Human

Target

Gamma-secretase subunit APH-1A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology APH1A ELISA Kit (Human) (OKEH00431) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human APH1A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human APH1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human APH1A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

1.56-100ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

0.78 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

26 kDa

Specificity

Natural and recombinant Human Gamma-secretase subunit APH-1A

Protein Length

247

NCBI Gene Symbol

APH1A

Protein Name

Q96BI3

Gene Name URL

APH1A

Nucleotide Accession Number

NM_001077628.2

Price

Frequently Asked Questions

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