What is the catalog number for IL27 ELISA Kit (Human)?

The catalog number for IL27 ELISA Kit (Human) is OKEH00409-96W.

What sizes are available for IL27 ELISA Kit (Human)?

IL27 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is IL27 ELISA Kit (Human) in stock?

IL27 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

IL27 ELISA Kit (Human)

Selected Variant

OKEH00409-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Interleukin 27

Gene Aliases

IL-27; IL-27 p28 subunit; IL-27 subunit alpha; IL27A; IL27-A; IL-27A; IL-27-A; IL27p28; IL30; interleukin-27 subunit alpha; interleukin-30; p28.

Gene ID

246778

Accession Number

NP_663634.2

Reactivity

Homo sapiens, Human

Target

Interleukin-27 subunit alpha

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology IL27 ELISA Kit (Human) (OKEH00409) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human IL27 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human IL27 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human IL27 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

3.9 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

27 kDa

Specificity

Natural and recombinant Human Interleukin-27 subunit alpha

Protein Length

243

NCBI Gene Symbol

IL27

Protein Name

Q8NEV9

Gene Name URL

IL27

Nucleotide Accession Number

NM_145659.3

Price

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

Mucin 6 (MUC6) (MUC6/916), CF405S conjugate, 0.1mg/mL

BNC040916-500 1x 500 µL