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CPT1A ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Carnitine palmitoyltransferase 1A

Gene Aliases

Carnitine O-palmitoyltransferase 1, liver isoform; Carnitine O-palmitoyltransferase I, liver isoform; Carnitine palmitoyltransferase 1A; carnitine palmitoyltransferase 1A (liver) ; carnitine palmitoyltransferase I, liver; CPT I; CPT1; CPT1-L; CPTI-L; L-CPT1.

Gene ID

1374

Accession Number

NP_001027017.1

Reactivity

Homo sapiens, Human

Target

Carnitine O-palmitoyltransferase 1, liver isoform

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CPT1A ELISA Kit (Human) (OKEH00404) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CPT1A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human CPT1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CPT1A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

88 kDa

Specificity

Natural and recombinant Human Carnitine O-palmitoyltransferase 1, liver isoform

Protein Length

773

NCBI Gene Symbol

CPT1A

Protein Name

P50416

Gene Name URL

CPT1A

Nucleotide Accession Number

NM_001031847.2

Frequently Asked Questions

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