GSN ELISA Kit (Human)
Product Specifications
Gene Name
Gelsolin
Gene Aliases
Actin-depolymerizing factor; ADF; AGEL; brevin; epididymis secretory sperm binding protein; gelsolin.
Gene ID
2934
Accession Number
NP_000168.1
Reactivity
Homo sapiens, Human
Target
Gelsolin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology GSN ELISA Kit (Human) (OKEH00402) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human GSN antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Human GSN and incubated. The Human GSN found in the sample or standards competes with the biotinylated Human GSN for limited binding sites on the immobilized anti-Human GSN antibody. Excess unbound biotinylated Human GSN and sample or standard human GSN is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Human GSN captured in the well and inversely proportional to the amount of human GSN which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.22 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
85 kDa
Specificity
Natural and recombinant Human Gelsolin
Protein Length
782
NCBI Gene Symbol
GSN
Protein Name
P06396
Gene Name URL
GSN
CAS Number
7732-18-5
Nucleotide Accession Number
NM_000177.4
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