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GSN ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Gelsolin

Gene Aliases

Actin-depolymerizing factor; ADF; AGEL; brevin; epididymis secretory sperm binding protein; gelsolin.

Gene ID

2934

Accession Number

NP_000168.1

Reactivity

Homo sapiens, Human

Target

Gelsolin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology GSN ELISA Kit (Human) (OKEH00402) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human GSN antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Human GSN and incubated. The Human GSN found in the sample or standards competes with the biotinylated Human GSN for limited binding sites on the immobilized anti-Human GSN antibody. Excess unbound biotinylated Human GSN and sample or standard human GSN is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Human GSN captured in the well and inversely proportional to the amount of human GSN which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.22 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

85 kDa

Specificity

Natural and recombinant Human Gelsolin

Protein Length

782

NCBI Gene Symbol

GSN

Protein Name

P06396

Gene Name URL

GSN

Nucleotide Accession Number

NM_000177.4

Frequently Asked Questions

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