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CXCL10 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

C-X-C motif chemokine ligand 10

Gene Aliases

10 kDa interferon gamma-induced protein; C7; crg-2; C-X-C motif chemokine 10; gamma IP10; gIP-10; IFI10; INP10; interferon-inducible cytokine IP-10; IP-10; mob-1; protein 10 from interferon (gamma) -induced cell line; SCYB10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B10.

Gene ID

3627

Accession Number

NP_001556.2

Reactivity

Homo sapiens, Human

Target

C-X-C motif chemokine 10

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CXCL10 ELISA Kit (Human) (OKEH00401) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CXCL10 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for Human CXCL10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CXCL10 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

10 kDa

Specificity

Natural and recombinant Human C-X-C motif chemokine 10

Protein Length

98

NCBI Gene Symbol

CXCL10

Protein Name

P02778

Gene Name URL

CXCL10

Nucleotide Accession Number

NM_001565.3

Frequently Asked Questions

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