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AVP ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Arginine vasopressin

Gene Aliases

ADH; antidiuretic hormone; ARVP; AVP-NPII; AVRP; copeptin; neurohypophyseal; prepro-arginine-vasopressin-neurophysin II; prepro-AVP-NP II; vasopressin-neurophysin 2-copeptin; vasopressin-neurophysin II-copeptin; VP.

Gene ID

551

Accession Number

NP_000481.2

Reactivity

Homo sapiens, Human

Target

Vasopressin-neurophysin 2-copeptin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology AVP ELISA Kit (Human) (OKEH00396) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human AVP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AVP and incubated. The AVP found in the sample or standards competes with the biotinylated AVP for limited binding sites on the immobilized anti-human AVP antibody. Excess unbound biotinylated AVP and sample or standard AVP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AVP captured in the well and inversely proportional to the amount of AVP which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

10 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

17 kDa

Specificity

Natural and recombinant Human Vasopressin-neurophysin 2-copeptin

Protein Length

164

NCBI Gene Symbol

AVP

Protein Name

P01185

Gene Name URL

AVP

Nucleotide Accession Number

NM_000490.4

Frequently Asked Questions

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