PECAM1 ELISA Kit (Human)
Product Specifications
Gene Name
Platelet and endothelial cell adhesion molecule 1
Gene Aliases
CD31; CD31 antigen; CD31/EndoCAM; endoCAM; GPIIA'; PECA1; PECAM-1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1.
Gene ID
5175
Accession Number
NP_000433.4
Reactivity
Homo sapiens, Human
Target
Platelet endothelial cell adhesion molecule
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PECAM1 ELISA Kit (Human) (OKEH00395) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PECAM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for PECAM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PECAM1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
31 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
83 kDa
Specificity
Natural and recombinant Human Platelet endothelial cell adhesion molecule
Protein Length
738
NCBI Gene Symbol
PECAM1
Protein Name
P16284
Gene Name URL
PECAM1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_000442.4
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