OAS1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
2'-5'-oligoadenylate synthetase 1
Gene Aliases
(2-5') oligo (A) synthase 1;2',5'-oligo A synthetase 1;2-5A synthase 1;2-5A synthetase 1;2'-5'-oligoadenylate synthase 1;2'-5'-oligoadenylate synthetase 1, 40/46kDa;2'-5'-oligoisoadenylate synthetase 1; E16 (2'-5') oligo A synthetase; E18/E16; IFI-4; OIAS; OIASI; p46/p42 OAS.
Gene ID
4938
Accession Number
NP_001027581.1
Reactivity
Homo sapiens, Human
Target
2'-5'-oligoadenylate synthase 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology OAS1 ELISA Kit (Human) (OKEH00383) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OAS1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for OAS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OAS1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
1.56-100ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.74 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
46 kDa
Notes
Formerly GWB-SORFKB
Specificity
Natural and recombinant Human 2'-5'-oligoadenylate synthase 1
Protein Length
400
NCBI Gene Symbol
OAS1
Protein Name
P00973
Gene Name URL
OAS1
Nucleotide Accession Number
NM_001032409.1
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