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SLC30A8 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Solute carrier family 30 member 8

Gene Aliases

Solute carrier family 30 (zinc transporter), member 8; Solute carrier family 30 member 8; zinc transporter 8; zinc transporter ZnT-8; ZnT-8; ZNT8.

Gene ID

169026

Accession Number

NP_001166282.1

Reactivity

Homo sapiens, Human

Target

Zinc transporter 8

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology SLC30A8 ELISA Kit (Human) (OKEH00379) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human SLC30A8 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SLC30A8 and incubated. The SLC30A8 found in the sample or standards competes with the biotinylated SLC30A8 for limited binding sites on the immobilized anti-human SLC30A8 antibody. Excess unbound biotinylated SLC30A8 and sample or standard SLC30A8 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SLC30A8 captured in the well and inversely proportional to the amount of SLC30A8 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%

Sensitivity

0.12 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

41 kDa

Specificity

Natural and recombinant Human Zinc transporter 8

Protein Length

369

NCBI Gene Symbol

SLC30A8

Protein Name

Q8IWU4

Gene Name URL

SLC30A8

Nucleotide Accession Number

NM_001172811.1

Frequently Asked Questions

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