DEFB4A ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Defensin beta 4A, defensin beta 4B
Gene Aliases
BD-2; beta defensin 2; beta defensin-2; Beta-defensin 2; beta-defensin 4A; beta-defensin 4B; DEFB102; DEFB2; DEFB-2; DEFB4; DEFB4P; defensin, beta 2; defensin, beta 4; defensin, beta 4, pseudogene; HBD-2; SAP1; skin-antimicrobial peptide 1.
Gene ID
100289462, 1673
Accession Number
NP_001192195.1
Reactivity
Homo sapiens, Human
Target
Beta-defensin 4A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DEFB4A ELISA Kit (Human) (OKEH00376) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEFB4A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for DEFB4A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEFB4A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
Sensitivity
0.045 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
7 kDa
Specificity
Natural and recombinant Human Beta-defensin 4A
Protein Length
64
NCBI Gene Symbol
DEFB4A, DEFB4B
Protein Name
O15263
Gene Name URL
DEFB4A, DEFB4B
Nucleotide Accession Number
NM_001205266.1
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