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DEFB4A ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Defensin beta 4A, defensin beta 4B

Gene Aliases

BD-2; beta defensin 2; beta defensin-2; Beta-defensin 2; beta-defensin 4A; beta-defensin 4B; DEFB102; DEFB2; DEFB-2; DEFB4; DEFB4P; defensin, beta 2; defensin, beta 4; defensin, beta 4, pseudogene; HBD-2; SAP1; skin-antimicrobial peptide 1.

Gene ID

100289462, 1673

Accession Number

NP_001192195.1

Reactivity

Homo sapiens, Human

Target

Beta-defensin 4A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology DEFB4A ELISA Kit (Human) (OKEH00376) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEFB4A has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for DEFB4A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DEFB4A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%

Sensitivity

0.045 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

7 kDa

Specificity

Natural and recombinant Human Beta-defensin 4A

Protein Length

64

NCBI Gene Symbol

DEFB4A, DEFB4B

Protein Name

O15263

Gene Name URL

DEFB4A, DEFB4B

Nucleotide Accession Number

NM_001205266.1

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