SERPINA7 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Serpin family A member 7
Gene Aliases
Mutant serpin family A member 7; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7; serpin A7; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7; T4-binding globulin; TBG; TBGQTL; thyroxin-binding globulin; thyroxine-binding globulin.
Gene ID
6906
Accession Number
NP_000345.2
Reactivity
Homo sapiens, Human
Target
Thyroxine-binding globulin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SERPINA7 ELISA Kit (Human) (OKEH00373) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SERPINA7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for SERPINA7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SERPINA7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.33 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
46 kDa
Specificity
Natural and recombinant Human Thyroxine-binding globulin
Protein Length
415
NCBI Gene Symbol
SERPINA7
Protein Name
P05543
Gene Name URL
SERPINA7
Nucleotide Accession Number
NM_000354.5
Frequently Asked Questions
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