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SPTA2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Spectrin alpha, non-erythrocytic 1

Gene Aliases

Alpha-fodrin; alpha-II spectrin; DEE5; EIEE5; epididymis secretory sperm binding protein; fodrin alpha chain; NEAS; spectrin alpha chain, non-erythrocytic 1; spectrin, non-erythroid alpha chain; spectrin, non-erythroid alpha subunit; SPTA2.

Gene ID

6709

Accession Number

NP_001123910.1

Reactivity

Homo sapiens, Human

Target

Spectrin alpha chain, non-erythrocytic 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SPTA2 ELISA Kit (Human) (OKEH00364) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPTA2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for SPTA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SPTA2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.18 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

285 kDa

Specificity

Natural and recombinant Human Spectrin alpha chain, brain

Protein Length

2472

NCBI Gene Symbol

SPTAN1

Protein Name

Q13813

Gene Name URL

SPTAN1

Nucleotide Accession Number

NM_001130438.2

Frequently Asked Questions

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