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INTS3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Integrator complex subunit 3

Gene Aliases

C1orf193; C1orf60; INT3; integrator complex subunit 3; sensor of single-strand DNA complex subunit A; sensor of ssDNA subunit A; SOSS complex subunit A; SOSSA; SOSS-A.

Gene ID

65123

Accession Number

NP_075391.3

Reactivity

Homo sapiens, Human

Target

Integrator complex subunit 3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology INTS3 ELISA Kit (Human) (OKEH00363) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for INTS3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for INTS3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample INTS3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

30 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

118 kDa

Specificity

Natural and recombinant Human Integrator complex subunit 3

Protein Length

1043

NCBI Gene Symbol

INTS3

Protein Name

Q68E01

Gene Name URL

INTS3

Nucleotide Accession Number

NM_023015.3

Frequently Asked Questions

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