PLD2 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Phospholipase D2
Gene Aliases
Choline phosphatase 2; phosphatidylcholine-hydrolyzing phospholipase D2; phospholipase D2; PLD1C.
Gene ID
5338
Accession Number
NP_001230037.1
Reactivity
Homo sapiens, Human
Target
Phospholipase D2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PLD2 ELISA Kit (Human) (OKEH00347) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PLD2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for PLD2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PLD2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
0.31 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
106 kDa
Specificity
Natural and recombinant Human Phospholipase D2
Protein Length
933
NCBI Gene Symbol
PLD2
Protein Name
O14939
Gene Name URL
PLD2
Nucleotide Accession Number
NM_001243108.1
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC940149-100 1x 100 µL
CD106 / VCAM1 (1.4C3), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC401081-100 1x 100 µL
CD45RO (190-2F2.5), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC740181-500 1x 500 µL
CD59 (heavy chain of Protectin) (BRA-10G), CF740 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC401073-500 1x 500 µL
HLA-Aw32 & HLA-A25 (MHC I) (CATA-1), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC941680-500 1x 500 µL
CD45RA (Leukocyte Marker) (K4B5), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC940819-100 1x 100 µL
Caldesmon, HMW (h-Caldesmon) (h-CALD), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details