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TPSB2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Tryptase alpha/beta 1, tryptase beta 2

Gene Aliases

Epididymis secretory sperm binding protein; mast cell alpha II tryptase; mast cell beta I tryptase; mast cell tryptase beta II; mast cell tryptase beta III; testicular tissue protein Li 163; TPS1; TPS2; TPSB1; TPSB2; tryptase alpha II; tryptase alpha/beta-1; tryptase alpha-1; tryptase beta I; tryptase beta-1; Tryptase beta-2; Tryptase II; tryptase-1; Tryptase-2; tryptaseB; tryptaseC; tryptase-I; tryptase-III.

Gene ID

64499, 7177

Accession Number

NP_003285.2

Reactivity

Homo sapiens, Human

Target

Tryptase beta-2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TPSB2 ELISA Kit (Human) (OKEH00342) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TPSB2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for TPSB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TPSB2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

0.11 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

31 kDa

Specificity

Natural and recombinant Human Tryptase beta-2

Protein Length

275

NCBI Gene Symbol

TPSAB1, TPSB2

Protein Name

P20231

Gene Name URL

TPSAB1, TPSB2

Nucleotide Accession Number

NM_003294.3

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