ETFDH ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Electron transfer flavoprotein dehydrogenase
Gene Aliases
Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial; electron-transferring-flavoprotein dehydrogenase; ETF dehydrogenase; ETFQO; ETF-QO; ETF-ubiquinone oxidoreductase; MADD; mutant electron transfer flavoprotein dehydrogenase.
Gene ID
2110
Accession Number
NP_001268666.1
Reactivity
Homo sapiens, Human
Target
Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ETFDH ELISA Kit (Human) (OKEH00337) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ETFDH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ETFDH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ETFDH captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
30 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
68 kDa
Specificity
Natural and recombinant Human Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial
Protein Length
617
NCBI Gene Symbol
ETFDH
Protein Name
Q16134
Gene Name URL
ETFDH
Nucleotide Accession Number
NM_001281737.1
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog