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ETFDH ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Electron transfer flavoprotein dehydrogenase

Gene Aliases

Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial; electron-transferring-flavoprotein dehydrogenase; ETF dehydrogenase; ETFQO; ETF-QO; ETF-ubiquinone oxidoreductase; MADD; mutant electron transfer flavoprotein dehydrogenase.

Gene ID

2110

Accession Number

NP_001268666.1

Reactivity

Homo sapiens, Human

Target

Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ETFDH ELISA Kit (Human) (OKEH00337) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ETFDH has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ETFDH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ETFDH captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

30 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

68 kDa

Specificity

Natural and recombinant Human Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial

Protein Length

617

NCBI Gene Symbol

ETFDH

Protein Name

Q16134

Gene Name URL

ETFDH

Nucleotide Accession Number

NM_001281737.1

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