CAT ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Catalase
Gene Aliases
Cas1; Cat01; catalase; Catl; Cs-1; CS1.
Gene ID
24248
Accession Number
NP_036652.1
Reactivity
Rat, Rattus norvegicus
Target
Catalase
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology CAT ELISA Kit (Rat) (OKEH00330) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAT has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CAT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAT captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Sensitivity
0.312 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
60 kDa
Specificity
Natural and recombinant Rat Catalase
Protein Length
527
NCBI Gene Symbol
Cat
Protein Name
P04762
Gene Name URL
Cat
Nucleotide Accession Number
NM_012520.2
Frequently Asked Questions
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