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ALPI ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Alkaline phosphatase, intestinal

Gene Aliases

Alkaline phosphomonoesterase; glycerophosphatase; IAP; intestinal alkaline phosphatase; intestinal-type alkaline phosphatase; Kasahara isozyme.

Gene ID

248

Accession Number

NP_001622.2

Reactivity

Homo sapiens, Human

Target

Intestinal-type alkaline phosphatase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ALPI ELISA Kit (Human) (OKEH00324) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALPI has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ALPI is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALPI captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

1.56-100ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.98 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

57 kDa

Specificity

Natural and recombinant Human Intestinal-type alkaline phosphatase

Protein Length

528

NCBI Gene Symbol

ALPI

Protein Name

P09923

Gene Name URL

ALPI

Nucleotide Accession Number

NM_001631.4

Frequently Asked Questions

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