What is the catalog number for VAV3 ELISA Kit (Human)?

The catalog number for VAV3 ELISA Kit (Human) is OKEH00310-96W.

What sizes are available for VAV3 ELISA Kit (Human)?

VAV3 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is VAV3 ELISA Kit (Human) in stock?

VAV3 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

VAV3 ELISA Kit (Human)

Selected Variant

OKEH00310-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Vav guanine nucleotide exchange factor 3

Gene Aliases

Guanine nucleotide exchange factor VAV3; vav 3 guanine nucleotide exchange factor; vav 3 oncogene; VAV-3.

Gene ID

10451

Accession Number

NP_001073343.1

Reactivity

Homo sapiens, Human

Target

Guanine nucleotide exchange factor VAV3

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology VAV3 ELISA Kit (Human) (OKEH00310) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VAV3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for VAV3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VAV3 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

98 kDa

Specificity

Natural and recombinant Human Guanine nucleotide exchange factor VAV3

Protein Length

847

NCBI Gene Symbol

VAV3

Protein Name

Q9UKW4

Gene Name URL

VAV3

Nucleotide Accession Number

NM_001079874.1

Price

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