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CCL18 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

C-C motif chemokine ligand 18

Gene Aliases

Alternative macrophage activation-associated CC chemokine 1; AMAC1; AMAC-1; CC chemokine ligand 18; CC chemokine PARC; C-C motif chemokine 18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated) ; chemokine (C-C), dendritic; CKb7; DCCK1; DC-CK1; dendritic cell chemokine 1; macrophage inflammatory protein 4; MIP-4; PARC; pulmonary and activation-regulated chemokine; SCYA18; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated; Small-inducible cytokine A18.

Gene ID

6362

Accession Number

NP_002979.1

Reactivity

Homo sapiens, Human

Target

C-C motif chemokine 18

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CCL18 ELISA Kit (Human) (OKEH00302) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL18 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CCL18 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL18 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.077 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

10 kDa

Specificity

Natural and recombinant Human C-C motif chemokine 18

Protein Length

89

NCBI Gene Symbol

CCL18

Protein Name

P55774

Gene Name URL

CCL18

Nucleotide Accession Number

NM_002988.3

Frequently Asked Questions

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