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GUSB ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Glucuronidase beta

Gene Aliases

Beta-D-glucuronidase; beta-G1; beta-glucuronidase; BG; MPS7.

Gene ID

2990

Accession Number

NP_000172.2

Reactivity

Homo sapiens, Human

Target

Beta-glucuronidase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology GUSB ELISA Kit (Human) (OKEH00293) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human GUSB antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GUSB and incubated. The GUSB found in the sample or standards competes with the biotinylated GUSB for limited binding sites on the immobilized anti-human GUSB antibody. Excess unbound biotinylated GUSB and sample or standard GUSB is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GUSB captured in the well and inversely proportional to the amount of GUSB which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10U/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%

Sensitivity

0.065U/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

75 kDa

Specificity

Natural and recombinant Human Beta-glucuronidase

Protein Length

651

NCBI Gene Symbol

GUSB

Protein Name

P08236

Gene Name URL

GUSB

Nucleotide Accession Number

NM_000181.3

Frequently Asked Questions

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