SERPINA6 ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Serine (or cysteine) peptidase inhibitor, clade A, member 6
Gene Aliases
Cbg; corticosteroid-binding globulin; serine (or cysteine) proteinase inhibitor, clade A, member 6; serpin A6; transcortin.
Gene ID
12401
Accession Number
NP_031644.1
Reactivity
Mouse, Mus musculus
Target
Corticosteroid-binding globulin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SERPINA6 ELISA Kit (Mouse) (OKEH00288) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse SERPINA6 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SERPINA6 and incubated. The SERPINA6 found in the sample or standards competes with the biotinylated SERPINA6 for limited binding sites on the immobilized anti-mouse SERPINA6 antibody. Excess unbound biotinylated SERPINA6 and sample or standard SERPINA6 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SERPINA6 captured in the well and inversely proportional to the amount of SERPINA6 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Detection Range
3.12-200ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.85 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
45 kDa
Specificity
Natural and recombinant Mouse Corticosteroid-binding globulin
Protein Length
397
NCBI Gene Symbol
Serpina6
Protein Name
Q06770
Gene Name URL
Serpina6
Nucleotide Accession Number
NM_007618.3
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