Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

FGF21 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Fibroblast growth factor 21

Gene Aliases

Fibroblast growth factor 21.

Gene ID

26291

Accession Number

NP_061986.1

Reactivity

Homo sapiens, Human

Target

Fibroblast growth factor 21

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology FGF21 ELISA Kit (Human) (OKEH00283) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FGF21 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for FGF21 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FGF21 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

12 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

22 kDa

Specificity

Natural and recombinant Human Fibroblast growth factor 21

Protein Length

209

NCBI Gene Symbol

FGF21

Protein Name

Q9NSA1

Gene Name URL

FGF21

Nucleotide Accession Number

NM_019113.3

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

CD4 (EDU-2), CF568 conjugate, 0.1mg/mL
BNC680345-100 1x 100 µL

CD4 (EDU-2), CF568 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD46 (122.2), CF568 conjugate, 0.1mg/mL
BNC680175-500 1x 500 µL

CD46 (122.2), CF568 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD100 (Semaphorin-4D) (A8), CF405S conjugate, 0.1mg/mL
BNC040218-500 1x 500 µL

CD100 (Semaphorin-4D) (A8), CF405S conjugate, 0.1mg/mL

Sign In for Pricing
View Details
Cytokeratin 17 (E3), CF594 conjugate, 0.1mg/mL
BNC940158-500 1x 500 µL

Cytokeratin 17 (E3), CF594 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF647 conjugate, 0.1mg/mL
BNC472308-500 1x 500 µL

HSP27 (Heat Shock Protein 27) (rHSPB1/774), CF647 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD74 (LN-2), CF405S conjugate, 0.1mg/mL
BNC040056-500 1x 500 µL

CD74 (LN-2), CF405S conjugate, 0.1mg/mL

Sign In for Pricing
View Details