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ELANE ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Elastase, neutrophil expressed

Gene Aliases

Bone marrow serine protease; ELA2; elastase 2, neutrophil; elastase-2; GE; granulocyte-derived elastase; HLE; HNE; Human leukocyte elastase; leukocyte elastase; medullasin; NE; neutrophil elastase; PMN elastase; PMN-E; polymorphonuclear elastase; polymorphonuclear leukocyte elastase; SCN1.

Gene ID

1991

Accession Number

NP_001963.1

Reactivity

Homo sapiens, Human

Target

Neutrophil elastase

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ELANE ELISA Kit (Human) (OKEH00275) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ELANE has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ELANE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ELANE captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%

Sensitivity

31 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

29 kDa

Specificity

Natural and recombinant Human Neutrophil elastase

Protein Length

267

NCBI Gene Symbol

ELANE

Protein Name

P08246

Gene Name URL

ELANE

Nucleotide Accession Number

NM_001972.2

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