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KDR ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

Kinase insert domain receptor

Gene Aliases

Fetal liver kinase 1; FLK-1; FLK1 kinase insert domain receptor (a type III receptor tyrosine kinase) (VEGF receptor 2) ; FLK1 kinase insert domain receptor (VEGF receptor 2) ; kinase insert domain protein receptor; protein-tyrosine kinase receptor flk-1; vascular endothelial growth factor receptor 2; Vegfr-2.

Gene ID

25589

Accession Number

NP_037194.1

Reactivity

Rat, Rattus norvegicus

Target

Vascular endothelial growth factor receptor 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology KDR ELISA Kit (Rat) (OKEH00246) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KDR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for KDR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KDR captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

39.1 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

150 kDa

Specificity

Natural and recombinant Rat Vascular endothelial growth factor receptor 2

Protein Length

1343

NCBI Gene Symbol

Kdr

Protein Name

O08775

Gene Name URL

Kdr

Nucleotide Accession Number

NM_013062.1

Frequently Asked Questions

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