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VCAM1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Vascular cell adhesion molecule 1

Gene Aliases

CD106; CD106 antigen; INCAM-100; vascular cell adhesion protein 1.

Gene ID

7412

Accession Number

NP_001069.1

Reactivity

Homo sapiens, Human

Target

Vascular cell adhesion protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology VCAM1 ELISA Kit (Human) (OKEH00233) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for VCAM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for VCAM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample VCAM1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%

Sensitivity

0.039 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

81 kDa

Specificity

Natural and recombinant Human Vascular cell adhesion protein 1

Protein Length

739

NCBI Gene Symbol

VCAM1

Protein Name

P19320

Gene Name URL

VCAM1

Nucleotide Accession Number

NM_001078.3

Frequently Asked Questions

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