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LTA ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Lymphotoxin alpha

Gene Aliases

LT; LT-alpha; lymphotoxin-alpha; TNF superfamily, member 1; TNFB; TNF-beta; TNFSF1; TNLG1E; truncated lymphotoxin alpha; tumor necrosis factor beta; tumor necrosis factor ligand 1E; tumor necrosis factor ligand superfamily member 1.

Gene ID

4049

Accession Number

NP_000586.2

Reactivity

Homo sapiens, Human

Target

Lymphotoxin-alpha

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LTA ELISA Kit (Human) (OKEH00227) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LTA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for LTA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LTA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%

Sensitivity

12 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

22 kDa

Specificity

Natural and recombinant Human Lymphotoxin-alpha

Protein Length

205

NCBI Gene Symbol

LTA

Protein Name

P01374

Gene Name URL

LTA

Nucleotide Accession Number

NM_000595.3

Frequently Asked Questions

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