What is the catalog number for TIE1 ELISA Kit (Mouse)?

The catalog number for TIE1 ELISA Kit (Mouse) is OKEH00213-96W.

What sizes are available for TIE1 ELISA Kit (Mouse)?

TIE1 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is TIE1 ELISA Kit (Mouse) in stock?

TIE1 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

TIE1 ELISA Kit (Mouse)

Selected Variant

OKEH00213-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1

Gene Aliases

D430008P04Rik; TI; TIE; tie-; tie-1; tyrosine kinase receptor 1; tyrosine-protein kinase receptor Tie-1.

Gene ID

21846

Accession Number

NP_035717.2

Reactivity

Mouse, Mus musculus

Target

Tyrosine-protein kinase receptor Tie-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TIE1 ELISA Kit (Mouse) (OKEH00213) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TIE1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for TIE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TIE1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%

Sensitivity

0.085 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

125 kDa

Specificity

Natural and recombinant Mouse Tyrosine-protein kinase receptor Tie-1

Protein Length

1134

NCBI Gene Symbol

Tie1

Protein Name

Q06806

Gene Name URL

Tie1

Nucleotide Accession Number

NM_011587.2

Price

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