TNFSF8 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
TNF superfamily member 8
Gene Aliases
CD153; CD153 antigen; CD30 antigen ligand; CD30 ligand; CD30L; CD30-L; CD30LG; TNLG3A; tumor necrosis factor (ligand) superfamily, member 8; tumor necrosis factor ligand 3A; tumor necrosis factor ligand superfamily member 8; tumor necrosis factor superfamily member 8.
Gene ID
944
Accession Number
NP_001235
Reactivity
Homo sapiens, Human
Target
Tumor necrosis factor ligand superfamily member 8
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TNFSF8 ELISA Kit (Human) (OKEH00200) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNFSF8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TNFSF8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNFSF8 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Sensitivity
5.4 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
26 kDa
Notes
Formerly GWB-1455Y0
Specificity
Natural and recombinant Human Tumor necrosis factor ligand superfamily member 8
Protein Length
234
NCBI Gene Symbol
TNFSF8
Protein Name
P32971
Gene Name URL
TNFSF8
Nucleotide Accession Number
NM_001244
Frequently Asked Questions
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