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SELP ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Selectin P

Gene Aliases

CD62; CD62 antigen-like family member P; CD62P; GMP140; GMP-140; granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; GRMP; LECAM3; leukocyte-endothelial cell adhesion molecule 3; PADGEM; platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; PSEL; P-selectin; selectin P (granule membrane protein 140kDa, antigen CD62) .

Gene ID

6403

Accession Number

NP_002996

Reactivity

Homo sapiens, Human

Target

P-selectin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SELP ELISA Kit (Human) (OKEH00197) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SELP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SELP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SELP captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

62.5-4000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%

Sensitivity

28 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

91 kDa

Specificity

Natural and recombinant Human P-selectin

Protein Length

830

NCBI Gene Symbol

SELP

Protein Name

P16109

Gene Name URL

SELP

Nucleotide Accession Number

NM_003005

Frequently Asked Questions

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