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PDGFRA ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Platelet derived growth factor receptor alpha

Gene Aliases

Alpha platelet-derived growth factor receptor; alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140A; CD140a antigen; PDGFR2; PDGFR-2; PDGF-R-alpha; Platelet-derived growth factor alpha receptor; platelet-derived growth factor receptor 2; platelet-derived growth factor receptor alpha; platelet-derived growth factor receptor, alpha polypeptide.

Gene ID

5156

Accession Number

NP_006197

Reactivity

Homo sapiens, Human

Target

Platelet-derived growth factor receptor alpha

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PDGFRA ELISA Kit (Human) (OKEH00194) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDGFRA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDGFRA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDGFRA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

0.045 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

123 kDa

Specificity

Natural and recombinant Human Platelet-derived growth factor receptor alpha

Protein Length

1089

NCBI Gene Symbol

PDGFRA

Protein Name

P16234

Gene Name URL

PDGFRA

Nucleotide Accession Number

NM_006206

Frequently Asked Questions

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