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CCL4 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

C-C motif chemokine ligand 4, C-C motif chemokine ligand 4 like 1

Gene Aliases

ACT2; AT744.1; AT744.2; CC chemokine ligand 4L1; C-C motif chemokine 4; C-C motif chemokine 4-like; CCL4L; chemokine (C-C motif) ligand 4; chemokine (C-C motif) ligand 4-like 1, telomeric; G-26; G-26 T-lymphocyte-secreted protein; HC21; LAG1; LAG-1; lymphocyte activation gene 1 protein; macrophage inflammatory protein 1-beta; macrophage inflammatory protein-1b2; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta (1-69) ; Monocyte adherence-induced protein 5-alpha; PAT 744; Protein H400; SCYA2; SCYA4; SCYA4L; SCYA4L1; SCYA4L2; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b) ; small inducible cytokine A4-like; Small-inducible cytokine A4; Small-inducible cytokine A4-like; T-cell activation protein 2.

Gene ID

388372, 6351

Accession Number

NP_996890

Reactivity

Homo sapiens, Human

Target

C-C motif chemokine 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CCL4 ELISA Kit (Human) (OKEH00165) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%

Sensitivity

3.9 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

10 kDa

Specificity

Natural and recombinant Human C-C motif chemokine 4

Protein Length

92

NCBI Gene Symbol

CCL4, CCL4L1

Protein Name

P13236

Gene Name URL

CCL4, CCL4L1

Nucleotide Accession Number

NM_207007

Frequently Asked Questions

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