LEP ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Leptin
Gene Aliases
LEPD; leptin; leptin (murine obesity homolog) ; leptin (obesity homolog, mouse) ; OB; obese protein; obese, mouse, homolog of; obesity factor; OBS.
Gene ID
3952
Accession Number
NP_000221
Reactivity
Homo sapiens, Human
Target
Leptin
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LEP ELISA Kit (Human) (OKEH00150) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LEP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LEP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LEP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.625-40ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
0.26 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
19 kDa
Specificity
Natural and recombinant Human Leptin
Protein Length
167
NCBI Gene Symbol
LEP
Protein Name
P41159
Gene Name URL
LEP
Nucleotide Accession Number
NM_000230
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